Another work, regarding EpCAM(−) detection of CTC in NSCLC, proposed a pre-enrichment step using hematopoietic cell depletion, followed by a multiparametric fluorescence analysis, comprising pan-cytokeratin, EpCAM, N-cadherin (specific of mesenchymal cells), and CD133 (stem-like marker). The gene discussed is EPCAM; the disease is non-small cell lung carcinoma.