INS and Glucose intolerance: Collectively, it is indicated that HFD resulted in impaired glucose tolerance in SAMP8 mice, and chia seed is able to reverse HFD induced glucose intolerance; SAMP8 mice at low fat diet has impaired insulin tolerance test compared to SAMR1-LFD group; HFD in SAMP8 mice further worsen the insulin tolerance, while chia seed is unable to reverse HFD induced insulin intolerance in SAMP8 mice.