AKT1 and neoplasm: Coincident with the results above, western blot analysis showed that PTEN as well as the epithelial markers E-cadherin and Claudin were increased but p-Akt and p-GSK3β and the mesenchymal markers N-cadherin, Vimentin with the EMT transcription factor Snail were decreased in the orthotopic tumor tissues of mice after fisetin treatment (Figures 6D,E).