KEGG pathway analysis identified several pathways that most significantly upregulated in CL4176 nematodes treated with brain extracts, some of which have been widely reported playing critical roles in AD pathological process, such as the ubiquitin mediated proteolysis, the TGF-β signaling pathway, and the mTOR signaling pathway (Figure 4B). The gene discussed is TGFB1; the disease is Alzheimer disease.