Analysis of rare variants in the regions of genes previously identified as related to AD by GWAS revealed genome-wide significant or suggestive evidence of association in established genes including TREM2, SORL1, and ABCA7. In addition, notable associations were observed with other genes in these regions not previously linked to AD including TREML4, SPPL2A, and AP4M1 (Supplementary Table S10). The gene discussed is SPPL2A; the disease is Alzheimer disease.