No significant increase in the IFNγ producing cell population was observed in three healthy individuals (example: healthy donor 1) in response to either the mutant peptide or wildtype peptides (Fig. 4A,B, and data not shown), suggesting that this MLH-1 germline mutant peptide is incapable of eliciting an immune response in an individual unaffected with LS-CRC. The gene discussed is MLH1; the disease is colorectal carcinoma.