To further validate that SOX2 upregulates PIK3CA expression thereby activating PI3K/Akt signaling pathways in nasopharyngeal carcinoma, we overexpressed PIK3CA in HNE-1 nasopharyngeal carcinoma cells with SOX2 shRNAs or a control shRNA. The gene discussed is SOX2; the disease is nasopharyngeal carcinoma.