The aberrant DNA methylation patterns in gene promoter are linked to a growing number of diseases, including cancer.38, 39 Recent studies have identified that HOXB13, a direct DNMT3B target gene, is hypermethylation at the upstream CpG island and functions as a tumor suppressor expression in the colon cancer and renal cell carcinoma.27, 40 In this study, by performing BSP assay, we found that the methylation level of HOXB13 promoter region was higher in HOXB13‐AS1‐upregulated cells compared to NC cells (Figure 4A,B). Here, DNMT3B is linked to malignant colon neoplasm.