Consistent with the mRNA expression in glioma tissues, the qRT‐PCR results showed that HOXB13‐AS1 mRNA was significantly upregulated in all tested human glioma cell lines ((U251, U87, SHG‐44, and SHG139), notably U251 and U87), compared with the normal human astrocytes cell line (Figure 1C). The gene discussed is HOXB13; the disease is central nervous system cancer.