Notably, the glycolytic signature is enriched in progressing melanomas after PD-1 blockade (Fig. 5g, p = 0.06, t-test, p = 0.02 when excluding stable disease) and is inversely associated with expression of the hot transcriptional signature (Rho = −0.44) that is associated with ICB response (Figs. 4 and 5h). The gene discussed is PDCD1; the disease is melanoma.