As we reported earlier36, the adherent forms of the studied HCT116 and HT29 CRC cell lines are characterized by 100%-enrichment in CD133+CD44+CD29+ CSCs, however, when the culture conditions were modified into sphere-forming environment, a decrease of CD133+ cells number by 39% and 59% in HCT116 and HT29 cell cultures, respectively, was found. Here, PROM1 is linked to colorectal carcinoma.