Finally, as irinotecan induces AKT and MEK phosphorylation in CRC cells (Fig. 1c), the interaction of the SN38, BKM120-MEK162 combination was evaluated in HCT116 cells using the SRB cytotoxicity assay and a three-dimensional dose matrix with increasing doses of the three drugs (five doses for BKM120, five doses for MEK162 and one dose for SN38) (Fig. 6a). This evidence concerns the gene MAP2K7 and colorectal carcinoma.