The receptor-transfected cells were stimulated either with the TxB cell hybridoma T2.A1 (CSPG4−, CEA−) or the human melanoma cell line A375M (CSPG4+, CEA−), and the production of the pro-inflammatory cytokines interleukin-2 (IL-2), tumor necrosis factor (TNF), and interferon-gamma (IFNγ) was characterized. This evidence concerns the gene TNF and melanoma.