ANLN and hepatocellular carcinoma: To further characterize whether ANLN responds to miR-15a or miR-16-1 through direct 3’ UTR interaction in HCC cells, we cloned a region of the wild-type ANLN 3’ UTR containing the putative miR-15a and miR-16-1 targets into a reporter plasmid downstream of the luciferase gene.