Here, we observed higher lesion size and bacterial loads when NADPH oxidase was deficient, and while LTB4 alone increased bacterial clearance in WT mice, LTB4 treatment did not reduce the infection areas or bacterial burdens, and both the apocynin co-treatment or gp91phox-/- mice showed similar results, further confirming that LTB4 increased bacterial killing by activating NADPH oxidase in vivo (Fig 6D and 6E). Here, CYBB is linked to infection.