We observed that the Tmem135 TG heart had significantly increased collagen compared to the WT heart at 6 months of age (Fig 1B; n = 4 WT, n = 4 TG, p<0.05 by t-test) indicating increased fibrosis, which is often associated with cardiac hypertrophy [28–30]. The gene discussed is TMEM135; the disease is cardiac hypertrophy.