For example, TRM derived from NSCLC and melanoma did not express membrane CTLA-4 (19, 35), and in ovarian cancer, PD-1+CD103+CD8+ T cells exhibited a weak expression of other exhaustion-associated markers, such as CTLA-4, LAG-3, and TIM-3 (32). This evidence concerns the gene CTLA4 and ovarian carcinoma.