Of the 72 cases with IHC analysis, 43 cases were positive in both nucleus and cytoplasm, 1 case with only cytoplasmic expression, and 2 cases with exclusive nuclear expression (the expression pattern of Notch3 showed in Supplementary FigureS4), while 26 breast cancer cases were negative for expression of Notch3. This evidence concerns the gene NOTCH3 and breast carcinoma.