CALR and malignant colon neoplasm: In addition, we examined the presence of CRT-binding sites on a human AML cell line HL60 and a human colon cancer cell line SW620, and found that their endogenous CRT was either expressed at a low level or limited to the perinuclear regions while a significant portion of PHA-L staining were observed on the cell surface (Fig. 4e, f).