After confirming the presence of SIRT1 on target gene promoters, we proceeded to investigate whether SIRT1 specifically interacts with histone H3 acetylated marks on those promoters by performing ChIP followed by re-ChIP assays on 110 breast tumors from all 5 molecular subtypes and their 110 matched normal tissues (n = 26 luminal A, n = 24 luminal B (HER2-), n = 20 luminal B (HER2+), n = 15 HER2-enriched and n = 25 triple-negative). Here, SIRT1 is linked to breast neoplasm.