SIRT1 and breast carcinoma: In order to determine whether HDAC SIRT1 interacts with histone H3 acetylated epi-marks in human breast cancer, we began by performing chromatin immunoprecipitation (ChIP) assays of SIRT1 on 50 breast tumors and their 50 matched normal tissues (n = 10 tumors for each of the 5 molecular subtypes).