EZH2 and breast cancer: To further analyze SIRT1 epigenetic role in sporadic breast cancer, we opted to study SIRT1 interaction with its H3 acetylated targets by carrying out ChIP and re-ChIP assays on 6 breast-cancer related genes: AR, BRCA1, ERS1, ERS2, EZH2 and EP300. The targeted genes play major roles in breast cancer carcinogenesis, either by stimulating breast tumors development and tumor progression, such is the case for oncogenes AR [41], ERS1 [42], EZH2 [43] and EP300 [44], or having anti-proliferative properties such as tumor suppressor genes BRCA1 [45] and ERS2 [46].