The samples were analyzed by real-time q-PCR targeting the promoters of the breast cancer-related gene panel: AR, BRCA1, ERS2, ERS1, EZH2, and EP300. The results showed a significant increase of both targeted epi-marks H3k4ac and H3k9ac on all 6 gene promoters across all 5 transfected cell lines in comparison to non-transfected control lines (Figures 9 and 10). This evidence concerns the gene BRCA1 and breast cancer.