TP53 and cancer: As expected, Scahigh and Scalow cells showed very different gene expression (“Sca-signature”, 3589 genes) and were enriched in pathways involved in fate determination, plasticity, and cancer, such as EMT, p53 signalling, Wnt/beta-catenin, and Notch pathway (Fig. 4h, i and Supplementary Fig. 3b).