To further verify the relationship between O-GlcNAcylation and the metastasis of CRC, we selected 15 cases of CRC patients with lymph node metastases and detected the expression of O-GlcNAcylation, OGT and OGA in the adjacent tissues, tumor tissues, and lymph node metastases, respectively (Fig. 1e). This evidence concerns the gene OGT and neoplasm.