Neither control nor ischemic CD163+ BAMs showed expression of genes encoding for typical microglial proteins, like Sall1. Among the top genes down-regulated after ischemia we found the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) glutamate receptor Gria2, genes related to subcellular organization, like Syne2 and Fam65b, and genes of the extracellular matrix, such as Adam23, certain growth factor receptors, like Egfr, and genes related to immune responses particularly associated to inhibition of leukocyte activation, such as Siglec5 (Additional file 1: Table S2). The gene discussed is SIGLEC5; the disease is ischemia.