To detect HER3-specific antibodies that could recognize membrane-associated HER3, binding of vaccine-induced antibodies (VIA) in mouse serum was first tested using a cell-based ELISA against a murine triple negative breast cancer cell line (4 T1) transduced to express HER3 (4 T1-HER3) (Fig. 1a). Mice vaccinated with Ad-HER3 had serum titers > 1:800 against the 4 T1-HER3 cells, in contrast to the mice receiving control Ad-LacZ and Ad-GFP vaccines, which only displayed background levels of binding (Fig. 1a). The gene discussed is ERBB3; the disease is triple-negative breast carcinoma.