Taking together, we speculated that overexpression of JARID1B promotes NSCLC cell proliferation, cell motility, invasiveness, and tumorsphere formation in vitro, not only allude to a novel role of JARID1B in the modulation of EMT and CSCs-like phenotype in NSCLC cells, but also give a mechanistic insight into the clinical observations that NSCLC patients with stronger JARID1B expression are more prone to have metastasis and significantly shorter overall survival. This evidence concerns the gene KDM5B and non-small cell lung carcinoma.