Overexpression of astroglial TLR2 significantly increased astroglial α-synuclein internalization as well as neuron-to-astrocyte α-synuclein transmission in in vitro synucleinopathy model system, while those were inhibited by anti-TLR2 treatment and TLR2 gene knockdown. The gene discussed is TLR2; the disease is synucleinopathy.