In view of the important role of the PI3K/Akt/mTOR pathway in autophagy regulation [27] and the role of Src in activating the PI3K/Akt pathway [11, 28], to determine how TSSC3 mediated autophagy in osteosarcoma, we first measured Src, Akt, mTOR, and their phosphorylation levels in TSSC3-overexpressing MTF and SaOS2 cells. Here, MTOR is linked to osteosarcoma.