Next-generation HLA sequencing allowed to us to determine that the DRB1*14:54-DQB1*05:03-carrying haplotypes in achalasia patients were DRB1*14:54:01-DQA1*01:04:01-DQB1*05:03:01-DPA1*01:03:01. The gene discussed is HLA-DQA1; the disease is Achalasia.