To further assess whether SENP3 is involved the regulation of Treg cell-mediated tumor immunosuppression by ROS, we carried out a side-by-side comparison using tumor-bearing Senp3+/+Foxp3-Cre, Senp3fl/flFoxp3-Cre, and Rag1−/− mice treated with NAC. Here, SENP3 is linked to neoplasm.