To confirm the effect of NAC on the stability and function of Treg cells, we injected Treg cells from Senp3+/+Foxp3-Cre and Senp3fl/flFoxp3-Cre (CD45.2+) mice pretreated with DMSO or NAC into tumor-bearing B6.SJL (CD45.1+) mice using CD45.1 and CD45.2 markers to clearly separate the host effect of T effector cells and donor Treg cells. The gene discussed is SENP3; the disease is neoplasm.