Downregulation of either Scn8a, Actn1, Srd5a, NFkB2 or Neurl1b interferes with the capability of cells to form: (a) colonies in anchorage-independent assays (Fig. 7b); (b) foci in anchorage-dependent assays, as revealed by a significant smaller foci size even if numbers were similar (Fig. 7c); (c) tumour spheres when cells were grown in self-renewal conditions (Fig. 7d); (d) tumours in nude mice xenografts (Fig. 7e). The gene discussed is ACTN1; the disease is neoplasm.