3a). Induction of nfP2X7 in RPMI-8226 cells requires the presence of functional P2X7 at the cell surface as demonstrated by L4-binding data (Supplementary Figure. 3b). ATP treatment leads to a rapid decrease in WT P2X7 at the cell surface, which removes the drive for a further increase in nfP2X7. Other cellular stresses and danger-associated molecular patterns may also drive nfP2X7 exposure at the surface of cancer cells and are being investigated. The gene discussed is P2RX7; the disease is cancer.