We also focused on the known hotspot mutations in CLL, NOTCH1 p.P2514fs*411,12, SF3B1 p.K700E, p.G742D, p.K666 and p.H66212,13, and MYD88 p.L265P14, and calculated the average depth of coverage in these specific genomic positions. The gene discussed is NOTCH1; the disease is B-cell chronic lymphocytic leukemia.