In one-round HIV-1 inGLuc infection assays, which was performed side-by-side, we observed a ~2-fold reduction of HIV-1 infection in IFNα2b-treated CD4+ T cells of both donors and that knockdown of IFITMs did not significantly rescue the IFN-mediated inhibition on HIV-1 infection (approximately 50%) (Figure 4F,I), suggesting that endogenous IFITMs in target CD+ T cells do not significantly contribute to IFN-mediated inhibition of HIV-1 entry. Here, CD4 is linked to HIV-1 infection.