HIV-1N57S was strikingly inhibited by KPNB1 depletion, and the ability of MX2 to reverse CypA induced inhibition of HIV-1N57S infection was abolished by KPNB1 depletion (Figure 8, Figure 8—figure supplement 5, Figure 9A, Figure 9—figure supplement 1B–C, and Figure 11C). Here, KPNB1 is linked to infection.