Although checklists and templates are especially useful in reminding pathologists to observe macroscopic features and microscopic information on routine hematoxylin and eosin–stained slides and to record information they have already observed (eg, margin status; tumor size, type, and grade; lymph node status; and lymphovascular invasion), logistical, technical, and economic factors have a greater effect on limiting the use of IHC assays of ER, PR, and HER2 status. This evidence concerns the gene ERBB2 and neoplasm.