To test the tumor suppressive function of ANKDD1A, we analyzed the growth characteristics and invasion ability (the upper chambers containing Matrigel coating mean that this assay measures an invasion phenotype but not migration phenotype of cells) in cells stably transduced with lenti-NC-GFP or lenti-ANK-GFP (Fig. 2d, e). The gene discussed is ANKDD1A; the disease is neoplasm.