Similarly, using high-resolution bisulfite genomic sequencing to analyze the methylation state in 33 CpG sites within the CpG island of the ANKDD1A gene, we found that there were more (20/33) methylated CpG sites in the ANKDD1A gene promoter in glioma compared to normal brain tissues (Fig. 1f). The gene discussed is ANKDD1A; the disease is central nervous system cancer.