Among the three available checkpoint inhibitors for advanced NSCLC patients, the PD-1 inhibitor pembrolizumab has been approved as a first-line therapy in patients with tumors harbouring PD-L1 expression ≥50% and as a second- or later-line treatment in patients with PD-L1 ≥1%7. The gene discussed is CD274; the disease is non-small cell lung carcinoma.