To investigate whether MACC1 expression is regulated by Wnt/β-catenin signaling pathway, we treated colon cancer cells (SW480 and HT-29) with either LiCl, a GSK3β inhibitor, or Wnt3a-conditioned media (CM), and monitored the expression levels of MACC1 by quantitative real-time reverse transcription-PCR (qRT-PCR) and immunoblot analysis. The gene discussed is MACC1; the disease is colonic neoplasm.