In accordance with our previous findings in breast tumors [23], the distribution of H3K9me3 was lower in colorectal TT of PD-1 (Fig. 4a) and H3K27me3 was lower in TT of the CTLA-4 (Fig. 4b) and TIM-3 (Fig. 4c) promoter regions compared with NT. This evidence concerns the gene HAVCR2 and breast neoplasm.