In contrast to tolerogenic cell death and other forms of tumor cell demise, ICD is characterized by three distinct molecular events which are necessary to prime and activate tumor-infiltrating dendritic cells: (1) translocation of calreticulin from the endoplasmic reticulum to the cell surface (i.e. ecto-calreticulin), (2) passive extracellular release of high-mobility-group box 1 (HMGB1) from the nucleus, and (3) extracellular release of ATP [87, 88]. This evidence concerns the gene CALR and neoplasm.