Overexpression of PAR1 in the PLC-PRF-5 model significantly increased tumor growth and lung metastasis, whereas knockdown of Twist1 in HepG2/M or PLC-PRF-5 cells overexpressing PAR1 inhibited the promotional effects on tumor progression by PAR1 (Fig. 6a and b). The gene discussed is TWIST1; the disease is neoplasm.