Upon high-fat diet (HFD)-induced obesity, percentage of the FSP1+/tdTomato+ SVF cells increased (I-WAT: 9.26% ± 0.28% [ND] versus 18.17% ± 1.36% [HFD]; E-WAT: 7.68% ± 0.96% [ND] versus 12.33 ± 0.67% [HFD]). The gene discussed is S100A4; the disease is obesity due to melanocortin 4 receptor deficiency.