Although cellular senescence is an irreversible arrest of cell proliferation, senescent cells remain metabolically active.24, 30 The expression profile of senescent cells, or the senescence‐associated secretory phenotype (SASP), is characterized and exhibits increased expression and secretion of various chemokines, cytokines, and other secretory proteins which are implicated in tumor progression and inflammatory responses.23 Matrine‐induced senescent U251 cells secreted lower levels of IL6, IGF1, angiogenin (ANG), TIMP2, and MIF and higher levels of CXXL10, FGF9, CK3CL1, MCP2, and TNFSF14. The gene discussed is TIMP2; the disease is neoplasm.