CD34 and chronic myelogenous leukemia, BCR-ABL1 positive: Furthermore, to verify the functional status of NF‐κB, transcriptional activity of the pathway was evaluated in CML cell lines (K562, Meg‐01, KCL‐22, Kasumi‐4), AML cell line HL60 and primary CD34+lin− cells from CML and NBM samples, according to Guzman and collaborators.22 Cells were cultured for 6 hours in the absence or presence of PTL or DMAPT at 7.5 μmol/L.