TNF and premenstrual tension: The validation cohort again mirrored the pilot cohort ICCS data with B cells derived from PMS patients secreting highest concentrations of TNF-α in comparison to RRMS, OIND, and non-inflammatory controls, although only comparisons to RRMS (Figure 4D; DM = −503.1 pg/mL, p = 0.0122) and non-inflammatory controls (Figure 4D; DM = −117.0 pg/mL, p = 0.0217) reached formal statistical significance.