Analysis of clinical samples in this study demonstrated that RIF1 expression was frequently up-regulated in EOC tissues and EOC cell lines compared with normal ovarian tissues and normal ovarian epithelial cell line and its overexpression associated with FIGO Stage (P < 0.001), overall survival (P = 0.0012) and progression-free survival (P < 0.001) of ovarian cancer patients. Here, RIF1 is linked to ovarian cancer.