To determine whether TMEM120B is a direct target of miR-92b-3p, we constructed luciferase reporter constructs by cloning the DNA segment-encoding part of the WT or mutant (which cannot bind miR-92b-3p) 3′UTR of TMEM120B mRNA downstream of the Renilla luciferase gene (Fig. 5A) and transfected them with miR-92b-3p mimics into the PCOS cumulus cells. This evidence concerns the gene TMEM120B and polycystic ovary syndrome.