CASP3 and cancer: Employing time lapse microscopy coupled with a battery of single cell assays, it was shown that treatment of cancer cell lines with an apoptosis-triggering agent resulted in caspase-3 activation, PS externalization (visualized by Annexin V staining), cell shrinkage, and other features of apoptosis (i.e., plasma membrane blebbing, cytoplasmic condensation, apoptotic body formation, mitochondrial fragmentation, mitochondrial cytochrome c release, DNA/chromatin condensation, and nuclear fragmentation).