Figure 3(c) shows that CD4+Foxp3+CTLA4+ TReg cells increase in frequency during sepsis as compared to sham (sham = 5.26 ± 0.81%, CLP = 9.31 ± 0.88%; p = 0.002) and that SIRT1 inhibition significantly reduces CD4+Foxp3+CTLA4+ TReg cell subpopulations (Figure 3(c)). The absolute cell counts of all the measured TReg subpopulations also decreased, but without statistical significance (Figure 3(d)). This evidence concerns the gene SIRT1 and Sepsis.