We evaluated the expression of phospho-CHK1 Ser317, phospho-CDC2 Tyr15, CDC2, and γ-H2AX in two ALL cases (1# and 6#) and the MNCs of one healthy donor (donor 4) after treatment with AZD-1775 at the same concentration reported above. This evidence concerns the gene CDK1 and acute lymphoblastic leukemia.