MC4R and obesity due to melanocortin 4 receptor deficiency: Following the above filtering, only a single homozygous variant was found that was shared between the two individuals: g.chr18: 58039459C < A, c.124 G > T, p.E42* in MC4R. No other variants (heterozygous or homozygous) in MC4R or in any other obesity-related gene were identified in any of the two subjects tested through whole exome sequencing.